List of abbreviations |
Vocabulary
of micros- copic anatomy specialist terms explained in English + German |
Every attempt was made to provide correct information and labelling, however any liability for eventual errors or incompleteness is rejected! |
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chromosomes
hypophsis (rat) |
detail: pairing in
metaphase |
skin (human)
anaphase |
detail 1 | detail 2 | detail 3 | human skin
prometaphase |
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Tonsilla pha-
ryngea (human) |
detail: formation of chro-
mosomes in prophase |
Tonsilla pharyngea
Prophase 2 (Mensch) |
detail 1: chromosome
formation |
detail 2 | detail 3 | Kinetochor liver
cell (rat) |
The number of chromosomes of organisms is species specific and constant.
With exception of the ova and spermatocytes
all other somatic cells of humans have, with exception of few multiploid
cells, a double set of chromosomes, i.e. they are diploid comprised of
46 chromosomes, 44 autosomes and 2 gonosomes. Autosomes are
all chromosomes bearing the genetic information for all non-sex specific
protiens of the body. One of the 2 chromosomes in each pair is from the
mother, the other from the father. These corresponding chromosomes that
carry the same genes are called homologous chromosomes. The gonosomes
which are also called heterosomes or sey chromosomes carry a large number
of genes with the information of sex-specific proteins and of proteins
required for the regulation and formation of primary and secondary sex
organs. There are 2 different gonosomes, the X-chromosome and the Y-chromosome.
While all cells in females have a pair of X-chromosomes,
cells of males show 1 X- and 1 Y-chromosome. In females
only the inforation of one X-chromosome is used whereas the second X-chromosome
is inactive and condensed as a X-chromatin or heterochromatic body (Barr
body). Such a Barr body is detectable in cells of the oropharyngeal
mucosa with the light- or electron microscope as a lump of heterochromatine
which has a diameter of 1 - 2 µm and which is attached to the inner
membrane of the cell nucleus.
The female reproductive cells (ova) are
haploid, i.e. posses only a half set of chromosomes in which all autosomes
are only present once plus one gonosome which always is an X-chromosome
(number of chromosomes: 22 + X). Male reproductive cells, i.e. spermatocytes
are also haploid with the difference that their gonosome may either be
an X- or an Y-chromosome. By fertilisation the ovum
gets the chromosomes of the spermatozoon
and the resulted fertilised ovum has a full (diploid) set of chromosomes
(either femal: 44 + XX or male: 44 + XY). The vast majority of cells is
euploid,
i.e. diploid with complete set of 46 chromosomes in the
cell
nucleus. In some organs, however, certain cells may have multiple sets
of chromosomes. This means they are polyploid. An example of polyploid
cells are hepatocytes. It is assumed that this is caused by the extremely
high metabolic activity. Usually these cells are tetraploid, i.e. have
a 4x set of chromosomes. Occasionally, hexaploid (6x) or octaploid (8x)
cells are encountered. In contrast to that tumor cells may be triploid
(3x) or pentaploid (5x) or other irregular sets of chromosomes.
The shape and the size as well as the bands of chromosomes in light
microscopic staining are constant allowing a classification using the size
and length of chromosome branches to the centromer. In a karyogram
all chromosomes are arranged according to their size using a micrograph
of usually a lymphocyte in light microsopic stain
after administration of a drug which stops mitosis in metaphase
when chromosomes are best visible. The best stain for visualisation of
the bands of chromosomes is orcein. The visible bands are caused by the
differences of chromatin fibil density in the regions of the chromosomes.
Further the relation of A-T to G-C pairs has an influence on stain. The
typical bands with size and location of the centromere allow to create
karyograms which are important for genetic investigations to detect abnormalities
e.g., trisomy (one chromosome is present 3x) or monosomy (present only
once). The micrographed chromosomes have 2 long processes, the chromatids
which are connected at the centromer as mentioned above. According to the
position of the centromer we can distinguish: metacentrisc chromosomes
(centromer in the centre: chromosomes 1,2,3,11,19,20,X), submetacentric
chromosomes (centromer not exactly in the centre: chromosomes 6,7,8,12),
acrocentric
chromosomes (centromer located close to the end: chromosomes 13,14,15,21,22,Y),
subacrocentric
chromosomes (centromer located closer to the end than to the centre: chromosomes
4,5,9,10,16,17,18). Since even in metacentric chromosomes the centre is
not absolutely exactly in the middle it is possible to always distinguish
2 longer and 2shorter arms of each chromosome. The longer ones are labelled
with q and the shorter ones with p. Further there are secondary
laces in the short arms of the acrocentric chromosomes 13, 14, 15,
21 and 22. These locations are the nucleolus-organisator centres
which are responsible for the formation of nucleoli.
The ends of the short arms of these chromosomes are called satellits
behind the incisure.
Chromosomes are electron-dense structures in a transmission
electron microscope which can only be seen in prometaphase,
metaphase
and anaphase of the mitosis. They are 2 to
10 µm in length and both chromatids are about 0.5 µm in thickness.
A clear determination which chromosome is regarded is not possible without
special procedures.
--> Euchromatine, heterochromatine,
synaptonemal
complex, cell nucleus, mitosis
--> Electron microscopic atlas Overview
--> Homepage of the workshop
Images, page & copyright H. Jastrow.